Further studies regarding the role of the plant-extract and its phyto-compounds on the gene and protein-level expression of a broad spectrum of inflammatory biomarkers would provide a clearer view on the anti-inflammatory aspects of the plant. 2015;10:e0135558. In the current study, rhizomes and fertile foliage fronds of this plant have been investigated comprehensively to assess their in vitro membrane stabilizing, thrombolytic and antioxidant properties. 4c). Article 2017;55:91741. Li SM, Tsai SE, Chiang CY, Chung CY, Chuang TJ, Tseng CC, et al. The AutoDock tool 1.5.6 was used for grid-based in silico molecular docking studies [48]. Crdeno A, Aparicio-Soto M, Montserrat-de la Paz S, Bermudez B, Muriana FJ, Alarcn-de-la-Lastra C. Squalene targets pro-and anti-inflammatory mediators and pathways to modulate over-activation of neutrophils, monocytes and macrophages. The haematological parameters including Hb content, total RBC, total WBC and total platelet count were measured and compared to the arthritic control group. According to Nadkarni (1976) and Dixit and Vohra (1984), the rhizome is used against typhoid fever. The limb region images were manually cropped from the digitized image and were analyzed [38]. Last Revised by: USDA NRCS National Plant Data Team. 2016;7:1822. Drynaria quercifolia 2015;3:1521. Inflammopharmacology. Asvakatri Do you know this herb by any other name ? Our in vitro and in vivo analysis of anti-inflammatory and anti-rheumatoid activity of DME showed promising results. Nature. 10c and d) and several hydrophobic interactions (at LEU 57, ILE 58, TYR 59, SER 60, GLN 61, TYR 119, GLY 121, GLY 122, TYR 151, ILE 155) reflected the second highest inhibitory binding energy (7.44 Kcal/Mol, inhibition constant 3.50M). Article Drynaria quercifolia BMC Compl Alternative Med. N Engl J Med. 1c). The ankle joints were surgically removed and placed in 10% formalin for fixation. WebDrynaria is used in treatments for tooth-related maladies, such as toothache and bleeding gums, as well as for tinnitus of the ears. The measurement of rat paw circumference was used to assess the degree of inflammation and to depict the possible therapeutic effects of DME on experimental rats. However, an extensive screening and identification of the major anti-inflammatory phyto-components is also required involving their isolation, purification, bio-availability assays. By using this website, you agree to our Drynaria quercifolia- Herbal Solution For Osteoarthritis Represents the interaction between the IL-6 and the inhibitor, Vitamin E, Alpha Tocopherol. Each phyto-component formed one H bond (both at TYR 151 residues) at target protein TNF- with a good binding energy (6.17 Kcal/Mol and4.77Kcal/Mol respectively) with several hydrophobic interactions (see Additional file 3). Med Sci. Benefits 5c and g), when compared to arthritic control groups. In China, Taiwan, Vietnam, Thailand, and Laos, the rhizomes of gu-sui-bu, Aglaomorpha fortunei (more frequently cited by Asian authors by its illegitimate synonym Drynaria fortunei), are commonly used to treat bone injuries. Drynaria quercifolia (commonly called Pankhiraj in Bengali/India) has been one of the pioneer medicinal plants which have a great medicinal value against various diseases. 2019;18:1628. First published in J. Bot. Click Here. McInnes IB, Schett G. The pathogenesis of rheumatoid arthritis. In this regard, the known phyto-compounds of Withania somnifera has shown interaction with different cytokines in in silico docking and in vivo animal models and downregulated the expressions of iNOS, TNF-, NF-B etc. PubChem substance and compound databases. 2019;47:D46474. 1.9 crystal structure of interleukin 6: implications for a novel mode of receptor dimerization and signaling. Paul S, Dutta T, Chaudhuri TK, Bhattacharjee S. Curative and protective properties of crude gel of Aloe vera from sub-Himalayan West Bengal in chronic and acute inflammatory rat models. 1970;16:90310. Conventional radiology in rheumatoid arthritis. The docking-based study reflected the binding affinity of the 7 chosen ligands to the active site region of COX-2, TNF- and IL-6 receptor as seen in heat map representation (Fig. Aglaomorpha (plant only, from day 0 to day 28. Drynaria quercifolia is belongs to the family of Polypodiaceae, is native to tropical areas of Africa, Asia, Australia and Oceania and cultivated mostly as a medicinal plant. When we compared with standard-drug group, all doses of DME (600g/ml, 800g/ml and 1000g/ml) significantly (p0.05) inhibited the denatured protein. Kim S, Thiessen PA, Bolton EE, Chen J, Fu G, Gindulyte A, et al. All data represented as meanSD of six animals and analyzed by One-way ANOVA followed by Dunnetts post hoc test where ***indicates p0.001, **indicates p0.01, and *indicates p0.05. In vitro membrane stabilizing, thrombolytic and antioxidant This due to the changes in plasma protein concentration and involvement of several inflammatory mediators like prostaglandins, bradykinin that increases the vascular permeability leads to the reduction of total protein and albumin levels [54]. 7e-h, lower panel) of all the groups also showed well-organized cellular structure and no abnormalities were seen in any groups. The goal of the present study was to validate the potent anti-inflammatory and anti-rheumatoid properties of the methanolic-extract of its rhizome using in vitro, in vivo and in silico strategies. Fractures. Curated and maintained by: USDA NRCS National Plant Data Team. The total run time of the program was 50min. Weininger D. SMILES, a chemical language and information system. 1998;19:163962. The animals received a booster dose of 0.1ml of FCA on the 14th day. Treatment with DME also normalized the haematological (RBC, WBC, platelet counts and hemoglobin contents) and biochemical parameters (total protein, albumin, creatinine and ceruloplasmin) significantly (p<0.05), which were further supported by histopathological and radiological analyses. 2018;46:W3637. SMILES server was utilized for converting the. EMBO J. The docking results were analyzed using Discovery Studio Visualizer software. Used for treating. 4. 2013;16:82147. New methyl 5-(halomethyl)-1-aryl-1H-1, 2, 4-triazole-3-carboxylates as selective COX-2 inhibitors and anti-inflammatory agents: design, synthesis, biological evaluation, and docking study. 2015;74:4809. CAS 5c and g) groups, the joint space appeared to be normal and soft tissue swelling was decreased compared to arthritic control groups. At the end of the incubation, the reaction mixtures were centrifuged at 1300g for 3min at room temperature and the absorbance (OD) of the supernatant was measured at 540nm. The FCA-induced arthritis model in rats is one of the most used models to study the anti-rheumatic properties of various compounds as it provides similar clinical and immunological characteristics to human RA [36, 52, 53]. In the current study, rhizomes and fertile foliage fronds of this plant have been investigated comprehensively to assess their in vitro membrane stabilizing, thrombolytic and antioxidant properties. Cell and Molecular Biology Laboratory, Department of Zoology, University of North Bengal, Raja Rammohunpur, Darjeeling, West Bengal, 734013, India, Debabrata Modak,Subhashis Paul,Sourav Sarkar&Soumen Bhattacharjee, Department of Bioinformatics, University of North Bengal, Darjeeling, West Bengal, 734013, India, You can also search for this author in Figure 7. represents the interaction between the TNF- and the inhibitor, Squalene. Prasanna G, Chitra M. In vitro antioxidant activity of Drynaria quercifolia L. rhizome. Clin Chem. Then the wooly brown scales of the rhizomes were removed and the clean rhizomes were shade dried at room temperature for 2weeks to obtain about 760g dry sample. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. BENEFITS, USES IN INDIVIDUAL DISEASES. 2020;28:13951. DM, SP and SS performed the in vitro and in vivo experiments and analyzed the data; DM performed the in silico experiments and interpreted the data; DM prepared and revised the manuscript; Interpretation of the experimental outcome, critical revision of the manuscript and approval of the manuscript for publication were done ST and SB. These radiographic changes are further supported by histopathological analysis of ankle-joints of the experimental rats. Prasanna G, Anuradha R. A comprehensive review on Phytopharmacological activities of Drynaria quercifolia L. Int J Pharmacogn Phytochem Res. Figure 2. Chicken egg albumin was used as a protein source and the experiment was carried out in triplicate sets (per dose). TNF- and IL-6 are two apex cytokines contributing to the progression of inflammation whereas, COX-2 elevates the inflammatory pain through prostaglandin production [10]. 2012;6:257680. Histological sections of rat paw (ankle) joints obtained from different experimental groups. The sections of kidneys (Fig. A novel mechanism of cyclooxygenase-2 inhibition involving interactions with Ser-530 and Tyr-385. The results of the docking study provide validation of anti-inflammatory activity. In vitro membrane stabilizing, thrombolytic and antioxidant Phytochemicals identified in GC-MS analysis were selected for molecular docking studies. It can be either epiphytic (when growing on trees) or epipetric (when growing on rocks) in nature. Drynaria quercifolia is a medicinal fern belongs to the family polypodiaceae. The goal of the present study was to validate the potent anti-inflammatory and anti-rheumatoid properties of the methanolic-extract of its rhizome using in vitro, in vivo and in silico strategies. Sato K, Tsuchiya M, Saldanha J, Koishihara Y, Ohsugi Y, Kishimoto T, et al. Data Documentation. Validating potent anti-inflammatory and anti-rheumatoid Another phyto-component, Phenylacetic acid showed 4.36 Kcal/Mol binding energy with the most H bond formation (at LYS 98, PRO 117, TYR 119 residues) at the target protein TNF- and showed a good binding energy (5.72 Kcal/Mol) with the target protein COX-2. Animals were acclimatized for 2 weeks before the study. Paw circumferences were measured at regular intervals of 2 to 3days from the day of arthritis induction, using the formula 2[(A2+B2)/2], where A and B were the measures of paw circumferences at two different axis lengths each placed at right angle to the other; one at dorsal-plantar side and the other at right-left side [36]. Squalene also formed several hydrophobic interactions (at ALA 199, ALA 202, GLN 203, THR 206, HIS 207, PHE 210, LYS 211, THR 212, GLN 289, VAL 291, ASN 382, HIS 386, TRP 387, HIS 388, LEU 390 and LEU 391 residues) with the target protein by covering the active site at tyrosine 385 residue). Briefly, the reaction mixture (5ml) consisted of 0.2ml of egg albumin, 2.8ml of phosphate-buffered saline (PBS, pH6.4) and 2ml of varying concentrations of DME resulting in the final concentrations of 600, 800, and 1000g/ml. WebThe rhizome is reported to have anti-fertility, anti-inflammatory and antipyretic, antimicrobial, antioxidant, wound healing, bone regenerative activity and various other activities of traditional and medicinal uses. Validating potent anti-inflammatory and anti-rheumatoid Google Scholar. Figure 12. Inflammatory responses and inflammation-associated diseases in organs. Bose D, Roy JG, Mahapatra SD, Datta T, Mahapatra SD, Biswas H. Medicinal plants used by tribals in Jalpaiguri district, West Bengal, India. indicates p0.001 and indicates p0.01. Both infectious and non-infectious stimuli can trigger an inflammatory pathway or acute inflammatory response that contributes to restoring physiological homeostasis. Burley SK, Berman HM, Bhikadiya C, Bi C, Chen L, Costanzo LD, et al. Stock erythrocyte suspension (100l) was added to each tube, and after gentle mixing, the tubes were incubated at 37C for 1h. After incubation, the reaction mixture was centrifuged for 3min at 1300g at room temperature and the absorbance (OD) of the supernatant was measured at 540nm. The main images represent sections observed under 10X magnification; inset represents 40X magnified images. 4b), which may indicate the suppressive activity of DME on the inflammatory mediators. In China, Taiwan, Vietnam, Thailand, and Laos, the rhizomes of gu-sui-bu, Aglaomorpha fortunei (more frequently cited by Asian authors by its illegitimate synonym Drynaria fortunei), are commonly used to treat bone injuries. 6b) and high dose (Fig. The old solution was replaced with a fresh solution every 5days till optimal decalcification. BENEFITS, USES IN INDIVIDUAL DISEASES. Figure 11. Computational proteinligand docking and virtual drug screening with the AutoDock suite. The PLANTS Database includes the following 1 data sources of Drynaria quercifolia (L.) J. Sm. 4d), which shows its effects on tissue repair. Structural basis for selective inhibition of cyclooxygenase-2 by anti-inflammatory agents. Both the low dose (6.840.43) and high dose (6.980.19) treated DME group showed a significant (p0.05) increase of total protein level when compared with arthritic control (6.110.25) (Fig. Presently, the available treatment protocol involves the use of conventional corticosteroids, disease-modifying anti-rheumatic drugs (DMARDs), such as methotrexate, hydroxychloroquine; non-steroidal anti-rheumatic drugs (NSAIDs), such as indomethacin, ibuprofen, aspirin, etc. The whole plant of Drynaria quercifolia is anthelmintic, pectoral, expectorant and tonic, and is used to treat chest and skin diseases and loss of appetite (Koushik and Dhiman, 1995). Anosike CA, Obidoa O, Ezeanyika LU. 2010;132:45660. J Pharm Pharm Sci. Inflammation. Tuberculosis. 3b). Last Revised by: USDA NRCS National Plant Data Team. Acute Oral toxicity - acute toxic class method, guideline no. Drynaria quercifolia L. (Family- Polypodiaceae) is a fern grows in Bangladesh used in traditional healing by the Garo tribe of Mymensingh district. Represents the interaction between the COX-2 and the inhibitor, 9,12-Octadecadienoic acid (Z,Z)-, methyl ester_Methyl Linoleate. 2014;20:42840. - basket fern P: Drynaria quercifolia (L.) J. Sm. Data Source. Shin J, Yang SJ, Lim Y. Gamma-tocopherol supplementation ameliorated hyper-inflammatory response during the early cutaneous wound healing in alloxan-induced diabetic mice. Diclofenac sodium (Novartis India Ltd., India) at the final concentration (100g/ml) was used as a standard drug. 6d), contrastingly, irregular cartilage lining (white arrow) was observed along with the cartilage being highly infiltrated by the immune cells (black arrow) due to severe inflammatory arthritis. The goal of the present study was to validate the potent anti-inflammatory and anti-rheumatoid properties of the methanolic-extract of its rhizome using in vitro, in vivo and in silico strategies. All the statistical analyses were performed using Graph Pad Prism Version 7.00 for Windows (GraphPad Software Inc., San Diego, USA). A solution of 3% HCl was used for decalcification [36]. An additional file shows all structures in 3D (see Additionalfile4). Article Drynaria quercifolia No. It also has antioxidant properties and can help fight oral bacteria. F1000Prime Rep. 2014;6:31. Bioorg Chem. basket fern. All the materials will be available for research purposes if requested to the corresponding author. Intestinal Worms . However, profound adverse effects are associated with the use of these agents, often leading to nephrotoxicity, gastrointestinal tract irritation, and hematological abnormalities and increased risk for cardiovascular diseases [14, 15]. Represents the interaction between the TNF- and the inhibitor, n Phenylacetic Acid. Benefits Scott DL, Wolfe F, Huizinga TW. Google Scholar. In case of creatinine level, DME did not produce any alteration in all the experimental groups, as we found a non-significant (F=0.56) difference when compared with each other (Fig. PLoS One. to each healthy rat of the vehicle group. Radiological analyses of right hind paw ankle joints of experimental rats. The injector was operated at 260C and the ion source temperature was set at 230C. Then the target protein was fed into the CASTp server for predicting the active sites [47]. In COX-2 docking studies, Squalene showed the highest binding affinity (9.59 Kcal/Mol) with a 0.094M inhibition constant whereas, Phenylacetic Acid showed the least binding affinity (5.72 Kcal/Mol) with a 64.12M inhibition constant. 2010;10:56. The initial infiltrating cells stimulate both Th1 and Th2 cell types; Th1 cells provide delayed type of hypersensitivity and Th2 involves B-cells in the inflammatory process [67]. Liver and kidney samples were also collected and fixed in 4% formalin. Ulcers. medicinal WebDrynariaquercifolia Search within this service search for plants and animals Back quercifolia Family Name: Polypodiaceae Synonyms: Aglaomorphaquercifolia Common Name: Oak Leaf Fern, Daun Kepala Tupai, Sakat Laipang, Semi Shade Little Water Occasional Misting Native to Singapore Suitable for Roadsides Ornamental Leaves Ferns and

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